Genetic Analyzer capillary electrophoresis for MLPA

Genetic Analyzer capillary electrophoresis for MLPA Dna Analyzer Description MLPA ( Multiplex Ligation-dependent Probe Amplification) method detects multiple copy number changes of genes or loci sites. Nowadays, MLPA is used to check large numbers of hereditary disorders and tumour profiling.Since Dutch Scientist Jan Schouten first invented it, the MLPA method was first published in 2002 'Nucleic Acid Research'. The principle of the MLPA is to apply the specific probe design targeting a region of interest on each sample DNA. MLPA method consists of the following steps:  Denaturation > Hybridization Ligation > PCR MLPA Amplificons Capillary Electrophoresis >Data Analysis We use sample DNA put into the thermocycler to separate the DNA strands. Then, the targeted DNA strand will be hybridized by adding up the Left and Right probe oligonucleotides. The ligase enzyme will bind the probe oligos up immediately to adjac…